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LC/ESI-MSn Screening for the Production of Neurotoxic Amino Acids in Cultured Cyanobacterial Isolate Nostoc MAC PCC 8009

Aminu Argungu Umar, Ahmad Ibrahim Bagudo.

Abstract
The non-protein amino acid β-methylamino-L-alanine (BMAA) is a neurotoxic agent that is produced by various strain of cyanobacteria. 2,4-diaminobutyric acid (2,4-DAB), and N-(2-aminoethyl) glycine (AEG) are the common isomers of BMAA. 2,4-DAB exhibit neurotoxic properties like BMAA. Various studies have shown that cyanobacteria produce BMAA and DAB, however, no studies have shown the detection of these amino acids using liquid chromatography-electrospray ionization ion trap mass spectrometry (LC/ESI-MSn) method. This study aimed to assess the occurrence of BMAA and its isomer 2,4-diaminobutyric acid (DAB) in axenic laboratory culture of cyanobacterial strain Nostoc MAC PCC 8009. Axenic laboratory cultures were harvested after 12 weeks of growth and non-protein amino acids were extracted by TCA and methanol extraction. Liquid chromatography-electrospray ionization ion trap mass spectrometry (LC/ESI-MSn) was employed to analyse the presence of BMAA and 2,4-DAB. Both BMAA and 2,4-DAB were detected in the axenic cultures.

Key words: Cyanobacteria, BMAA, DAB, LC-ESI-MSn, Nostoc, Derivatized


 
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Pubmed Style

Umar AA, Bagudo AI, . LC/ESI-MSn Screening for the Production of Neurotoxic Amino Acids in Cultured Cyanobacterial Isolate Nostoc MAC PCC 8009 .


Web Style

Umar AA, Bagudo AI, . LC/ESI-MSn Screening for the Production of Neurotoxic Amino Acids in Cultured Cyanobacterial Isolate Nostoc MAC PCC 8009 . http://www.equijost.com/?mno=91118 [Access: September 28, 2020].


AMA (American Medical Association) Style

Umar AA, Bagudo AI, . LC/ESI-MSn Screening for the Production of Neurotoxic Amino Acids in Cultured Cyanobacterial Isolate Nostoc MAC PCC 8009 .



Vancouver/ICMJE Style

Umar AA, Bagudo AI, . LC/ESI-MSn Screening for the Production of Neurotoxic Amino Acids in Cultured Cyanobacterial Isolate Nostoc MAC PCC 8009 .



Harvard Style

Umar, A. A., Bagudo, A. I. & (0) LC/ESI-MSn Screening for the Production of Neurotoxic Amino Acids in Cultured Cyanobacterial Isolate Nostoc MAC PCC 8009 .



Turabian Style

Umar, Aminu Argungu, Ahmad Ibrahim Bagudo, and . 0. LC/ESI-MSn Screening for the Production of Neurotoxic Amino Acids in Cultured Cyanobacterial Isolate Nostoc MAC PCC 8009 .



Chicago Style

Umar, Aminu Argungu, Ahmad Ibrahim Bagudo, and . "LC/ESI-MSn Screening for the Production of Neurotoxic Amino Acids in Cultured Cyanobacterial Isolate Nostoc MAC PCC 8009 ."



MLA (The Modern Language Association) Style

Umar, Aminu Argungu, Ahmad Ibrahim Bagudo, and . "LC/ESI-MSn Screening for the Production of Neurotoxic Amino Acids in Cultured Cyanobacterial Isolate Nostoc MAC PCC 8009 ."



APA (American Psychological Association) Style

Umar, A. A., Bagudo, A. I. & (0) LC/ESI-MSn Screening for the Production of Neurotoxic Amino Acids in Cultured Cyanobacterial Isolate Nostoc MAC PCC 8009 .





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