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2024, Vol: 11, Issue: 2
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Research Article
Online Published: 19 Sep 2020
 


LC/ESI-ITMS Detection of the Neurotoxic Amino Acids in Cultured Cyanobacterial Isolate Nostoc MAC PCC 8009

Aminu Argungu Umar, Ahmad Ibrahim Bagudo.


Abstract
The non-protein amino acid β-methylamino-L-alanine (BMAA), is a neurotoxic agent that is produced by various strain of cyanobacteria. 2,4-diaminobutyric acid (2,4-DAB), and N-(2-aminoethyl) glycine (AEG) are the common isomers of BMAA. 2,4-DAB exhibit neurotoxic properties like BMAA. Various studies have shown that cyanobacteria produce BMAA and DAB, however, no studies have shown the detection of these amino acids using liquid chromatography-electrospray ionization ion trap mass spectrometry (LC/ESI-ITMS) method. This study aimed to assess the occurrence of BMAA and its isomer 2,4-diaminobutyric acid (DAB) in axenic laboratory culture of cyanobacterial strain Nostoc MAC PCC 8009. Axenic laboratory cultures were harvested after 12 weeks of growth and non-protein amino acids were extracted by trichloroacetic acid (TCA) and methanol extraction. Liquid chromatography-electrospray ionization ion trap mass spectrometry (LC/ESI-ITMS) was employed to analyse the presence of BMAA and 2,4-DAB. Both BMAA and 2,4-DAB were detected in the axenic cultures, which confirms the production of these neurotoxic amino acids by cyanobacteria. Multiple stage mass analysis by ESI-ITMS using [M+H] + was useful to distinguish between the two isomers.

Key words: BMAA, Cyanobacteria, DAB, Derivatized, ESI-ITMS, Nostoc


 
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Pubmed Style

Umar AA, Bagudo AI. LC/ESI-ITMS Detection of the Neurotoxic Amino Acids in Cultured Cyanobacterial Isolate Nostoc MAC PCC 8009. Equijost. 2020; 7(2): 31-38.

Web Style

Umar AA, Bagudo AI. LC/ESI-ITMS Detection of the Neurotoxic Amino Acids in Cultured Cyanobacterial Isolate Nostoc MAC PCC 8009. https://www.equijost.com/?mno=91118 [Access: June 28, 2026].

AMA (American Medical Association) Style

Umar AA, Bagudo AI. LC/ESI-ITMS Detection of the Neurotoxic Amino Acids in Cultured Cyanobacterial Isolate Nostoc MAC PCC 8009. Equijost. 2020; 7(2): 31-38.


Vancouver/ICMJE Style

Umar AA, Bagudo AI. LC/ESI-ITMS Detection of the Neurotoxic Amino Acids in Cultured Cyanobacterial Isolate Nostoc MAC PCC 8009. Equijost. (2020), [cited June 28, 2026]; 7(2): 31-38.


Harvard Style

Umar, A. A. & Bagudo, . A. I. (2020) LC/ESI-ITMS Detection of the Neurotoxic Amino Acids in Cultured Cyanobacterial Isolate Nostoc MAC PCC 8009. Equijost, 7 (2), 31-38.


Turabian Style

Umar, Aminu Argungu, and Ahmad Ibrahim Bagudo. 2020. LC/ESI-ITMS Detection of the Neurotoxic Amino Acids in Cultured Cyanobacterial Isolate Nostoc MAC PCC 8009. Equity Journal of Science and Technology, 7 (2), 31-38.


Chicago Style

Umar, Aminu Argungu, and Ahmad Ibrahim Bagudo. "LC/ESI-ITMS Detection of the Neurotoxic Amino Acids in Cultured Cyanobacterial Isolate Nostoc MAC PCC 8009." Equity Journal of Science and Technology 7 (2020), 31-38.


MLA (The Modern Language Association) Style

Umar, Aminu Argungu, and Ahmad Ibrahim Bagudo. "LC/ESI-ITMS Detection of the Neurotoxic Amino Acids in Cultured Cyanobacterial Isolate Nostoc MAC PCC 8009." Equity Journal of Science and Technology 7.2 (2020), 31-38. Print.


APA (American Psychological Association) Style

Umar, A. A. & Bagudo, . A. I. (2020) LC/ESI-ITMS Detection of the Neurotoxic Amino Acids in Cultured Cyanobacterial Isolate Nostoc MAC PCC 8009. Equity Journal of Science and Technology, 7 (2), 31-38.




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